survey of mycotoxins in wheat from iran by hplc using immunoaffinity column cleanup

mycotoxins are small, toxic chemical products produced as secondary metabolites by a few fungal spices that readily colonise crops and contaminate them with toxins in the field or after harvest. in this study a liquid chromatographic method was developed for the simultaneous determination of aflatoxins (afs) (b 1 , b 2 , g 1 , and g2), ochratoxin a (ota) and zearalenone(zea) toxins in wheat. for this purpose, a total of 231 wheat samples were analyzed. mycotoxins were extracted and purified from the samples using immunoaffinity (iac) columns. 2.67% and 2.21% of examined wheat samples contained afb 1 and ota. the method was based on the extraction of afs, ota and zea finely ground wheat sample with 80% methanol and 60% acetonitrile solution, respectively. 2.60% and 2.16% of examined wheat samples contained afb 1 and ota. lod was 0.27, 0.04, 0.23, 0.04, 1.18 and 21.56 for afb 1 , afb 2 , afg 1 , afg 2 , ota and zea, respectively.considering these results, these special products should not be a health concern for these collectives; however, special attention needs on foods distribution in retail shops.